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Isoform-Specific Biomarker discovery and diagnostics
Presented at BIO 2005 Annual International Convention, June 19-22, 2005, Philadelphia Pennsylvania
Abstract
Luke V. Schneider, Michael P. Hall, William W. Chang, David Bomberger, Christine Hobson, John P. Wilson, Lane A. Clizbe, Robert Petesch, and Siamak Ashrafi
The clinical relevance of specific protein variants (or isoforms) is becoming increasingly clear, particularly in cancer diagnosis and treatment. However, the ability to discover, validate, and utilize protein isoforms is severely limited, particularly in the clinic. We present a new isoform-specific discovery platform (IDBEST™) and a robust, cost-effective, clinical diagnostic immunoaffinity-capillary zone electrophoresis platform (ICZE™) to make isoforms clinically useful.
Isotope-Differentiated Binding Energy Shift Tags (IDBEST™) utilize mass defect technology to combine affinity-enrichment or baiting of low abundance biomarkers with good sequence coverage for isoform detection by mass spectrometric detection. Isotopic versions of the tags allow quantitative comparison of specific proteins and isoforms between samples.
The Immunoaffinity-Enrichment Capillary Zone Electrophoresis (ICZE™) platform allows enrichment of a specific protein and quantitative ratiometric determination of all its isoforms at a cost competitive with clinical ELISA methods. ICZE™ also fits in the footprint of existing clinical lab sample handling and processing systems.
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